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![]() ![]() Once you grab one, head back to the fabricator, and make a cooked Peeper. These little buttheads will always swim ahead of you and then hang a quick right to get away, so anticipate this move. Once you craft filtered, drinkable water, go ahead and catch a Peeper fish. Although disinfected water is better, Airsack fish make for a decent substitute until you become more familiar with the terrain. Until you manage to find salt scattered around the ocean floor to craft disinfected water, start off with snatching Airsack fish to create filtered water. Pick up two Airsack (lung) fish and one Peeper fish. Now you will be able to breathe underwater for 100 seconds.Ĥ. Gather these items, head back into the pod, explore the Fabricator (crafting machine), create two glass with your quartz, and then craft your two oxygen tanks. Quartz is the white crystal you find lingering in bushes, in caves, or at the edge of the safe shallows. Titanium is the most basic element in Subnautica and can be found in the rocks attached to underwater landscapes or in the metal salvage lingering on the ocean floor (among other places). In order to craft the oxygen tanks, you will need two titanium and four quartz. You’re welcome!įrom the jump, you’ll want to breathe longer underwater and swim faster than your body allows, so you’re going to need two oxygen tanks to start. Boom! You are now a faster swimmer with far longer underwater breathing time to boot. Once you navigate past them, grab four of the glowing yellow bulbs (creepvine cluster sample), head back to your fabricator, craft two silicone rubber, and then craft your fins. ![]() For the most part, these aggressive fish will ignore you, but try to watch out for them regardless. You will also want to watch out for Stalkers, they look like white mini dragons with blue scales. If one sucks onto your arm, hit it by right-clicking until it comes off. Make sure to keep an eye out for little fish that have pink tails and red heads, these are called bleeders and are essentially the leeches of the ocean. Surrounding the safe shallows, you will see long “creepy” seaweed trees that rise all the way to the surface from the seafloor. If you don’t want to swim back to the Fabricator to reference crafting ingredients, press alt+tab and keep the Subnautica wiki open in your browser for quick reference. I cannot stress enough how important it is to learn the basics of the Fabricator when it comes to playing efficiently and enjoyably. Study the basic recipes and start developing a plan for what you need to collect. This fabricator is essentially your Minecraft version of a crafting table. In the escape pod, you will find a wall-mounted Fabricator. With those important notes out of the way, let’s start with quick beginner’s tips: For hoarders like myself, I crafted three storage lockers surrounding the pod in the event that I had something good but didn’t want to drop it. If you haven’t created your own seabase yet, there is a small storage unit available on the bench inside the crash pod and you can also craft floatable storage lockers. Otherwise, your inventory will clutter up fairly quickly. With that in mind, make sure to only pick up these items if you need them right away. SO DON’T BE SCARED!Īlso, take note that certain items will occupy more space in your inventory, such as creepvine cluster samples, metal salvage, creature eggs, and handheld equipment. No one in the safe shallows will attack you and for the most part, it’s the “kiddy pool” of the ocean. Here there is plenty of fish to eat, quartz, titanium, and other basic crafting ingredients. The most habitable area available is the one where you land, the safe shallows. By applying the following tips, you will be well on your way to navigating this new world efficiently, enjoyably, and of course - with style.įor starters, it is important to note a few things about Subnautica. ![]() With so much at your disposal, the descent into this unique world takes quite a bit of time and resource management to ensure success. The extraterrestrial planet of Subnautica is large and filled with all sorts of flora, fauna, and unique challenges for even the most akin survival experts to overcome. ![]() ![]() (Doctor Manhattan’s reasons for not doing so have to do with his ability to see all time, including the future, at once, and can be summed up as: “He doesn’t save the world from evil because in the future he won’t save the world from evil.” His path is predetermined, which is frustrating from a narrative perspective.)Īnd every character who seeks to steal Doctor Manhattan’s power is ultimately shown to be evil, which complicates the message of the final scene when Angela consumes the egg into which Doctor Manhattan transferred his powers and tests whether she has gained superpowers by preparing to walk on water. In the final episode, many characters justifiably point out that Doctor Manhattan should have used his powers to stop global warming or destroy all the nuclear weapons in the world. The series seems decidedly mixed on whether anyone should be endowed with god-like abilities. And in the penultimate episode, Doctor Manhattan discusses the “Which came first, the chicken or the egg?” paradox with Angela, explaining that both came to be at the same time. Later, Hooded Justice reaches into a boiling pot of water to retrieve an egg with his hand. ![]() ![]() ![]() Angela cracks eggs in the shape of the iconic smiley face that appears on the cover of the graphic novel when demonstrating how to bake to her children and their classmates in the first episode. The egg was always going to be an important symbol of life throughout the show. ![]() ![]() While staying in New York City, he bumped into John Lennon, deep in the throes of his “lost weekend” period. After achieving stardom with Ziggy Stardust, a management dispute and a series of lawsuits had left Bowie feeling cynical about his success. īowie was a star before “Fame,” but the second single off Young Americans raised his status even further. Wood, no stranger to soul through his work with Faces and Rod Stewart, provides the tasty fretwork to pull it together and set the stage for Young Americans a year later. He takes The Boss’ Dylan-indebted cascade of words and bends it to fit a sound that merges his glam present with his Philly soul future. With soon-to-be Rolling Stone Ronnie Wood handling lead guitar on this unused Diamond Dogs cut, Bowie gives a nod to the next great lyricist and lays a future foundation with his cover of Bruce Springsteen’s “Growin’ Up”. ![]() By 1974, Ziggy was coming to a close, and Bowie was ready for the next phase in his evolution. “Growin’ Up” featuring Ronnie Wood (1974)Ī folkie in the years before becoming glam-rock god Ziggy Stardust, Bowie was drawn to transcendent singer-songwriters (look no further than his 1971 tune “Song for Bob Dylan” for proof of that). From his partnership with Mick Ronson and the legendary Berlin triptych concocted with Brian Eno to assuming the mantle of producer on iconic albums by Mott the Hoople, Lou Reed, and Iggy Pop, some of Bowie’s most memorable and important moments were birthed through the spirit of collaboration. Along with seeking out new sounds, the Bowie legacy is intertwined with his knack for reaching out and creating with others. While Bowie will rightly be remembered as a brilliant solo artist who consistently pursued new inspiration, he would be the first to tell you that his musical odyssey was never conducted alone. Along the way, he gifted listeners with a slew of memorable, solitary personas: Ziggy Stardust, the Thin White Duke, the Man Who Fell to Earth, the Goblin King, and, in his stunning final act, the Blind Prophet. ![]() ![]() And, if you’ve missed anything, you can experience it all again here.ĭavid Bowie was a singular force, blurring gender and genre lines in a career that spanned over half a century. Keep checking back all week for more new and reshared content reflecting on our favorite Starman. Editor’s Note: We continue our celebration of the life and art of David Bowie with a look at 10 magical collaborations. ![]() ![]() ![]() To collect proteins effectively, an appropriate homogenization method should be selected which can quickly and effectively discharge the cell's intracellular components via the bursting of cell membranes. An effective step in the separation and purification of proteins seems to have a significant effect on the final observation of western blot analysis experiments. Effective sample preparation is a crucially important factor of provable western blot analysis. Therefore, care shall be exercised to optimise the high sensitivity and reproducibility from each step of the western blotting procedure. Īlterations could even affect the course at any step of the procedure. This is a multi-step procedure that normally involves preparation of samples (collection of protein and protein concentration measurement) through tissue or cells lysates, segregation of proteins via size from polyacrylamide gel by electrophoresis on sodium dodecyl sulphate (SDS), immobilisation of separate molecules in the membrane of nitrocellulose or polyvinylidene fluoride (PVDF), blockage of non-specific membrane proteins, testing of protein targets with specialised specific antibody, cells were incubated with secondary antibodies combined with fluorescent molecule labels or chemiluminescent signal detection reflecting antigen/antibody binding, protein band densitometry assessment utilising a software. It’s a technique that’s widely used in protein analysis. The western blot is a popular method for detecting post-translational protein changes as well as protein structure and it offers quantitative or semi-quantitative data on the protein structure in single or multiple biological samples. In this review, the different techniques of western blotting and their improvement in different stages have been discussed. This paper describes another very developed strategy available as well as demonstrated the correlation among Western blotting techniques of the next generation and their clinical implications. The advancements in various region of west blotting included in this analysis of transfer of protein and validation of antibody are described. Discovery of new in devices and higher susceptibility for western blots gives innovative opportunities to expand Western blot’s clinical relevance. New approaches, like automated microfluid western blotting, DigiWest, single cell resolution, microchip electrophoresis, and capillary electrophoresis, were all implemented to reduce the future conflicts linked with the western blot analysis approach. Because it is a sequential framework, differences and inaccuracies could even take place at any stage, decreasing this particular method's reproducibility and reliability. Western blotting is frequently employed in molecular techniques like Proteomics and Biology. ![]() ![]() ![]() Some shops are owned by real life friends of mine, some have gifted me products over the years that I love and some I discovered this year, particularly as I was searching for more Black owned businesses to support. With that in mind, I’d like to highlight a few of my favorite small businesses to shop for the holidays. Little Miss Lovely Floral Design & Event Decorating - Ocean City, Maryland Wedding Florist - Unique, one-of-a-kind floral arrangements for weddings. You can purchase my book or shop the Strand here.įull disclosure- there has been some controversy about how The Strand spent their PPE money, so please take this shout-out as a call to action to help the local shops in your neighborhood and other struggling small businesses. You can help your local book shops by buying from most of them online. The Strand and other shops in NYC have reached out to their patrons for help to keep their businesses afloat during these unprecedented times when foot traffic is so low. The Strand had given me the opportunity to succeed in NYC and my people had showed up. So that night, when I looked out into that crowd of both familiar and unfamiliar faces, holding my book up high, it was with the hugest sense of gratitude. I feared I would be speaking to an empty audience, and then on top of that, have to pay a huge bill to underline my failure. Some of us even had to change into sneakers so we didnt miss. A bunch of my friends had called to cancel throughout the day and I began to fear that Barnes and Noble was right. After dinner and speeches, guests spent the rest of the night dancing and laughing the night away. I remember being so nervous walking there that night. ![]() I agreed, knowing that I would have to buy all those books myself if nobody showed. The catch was that I had to guarantee I would sell a certain number of books. My publisher thought that was an even bigger long shot, but based on me being from the neighborhood, they agreed to do it. We're all about big moments in intimate settings, or as we like to say 'big events, small spaces'. The Strand is a beloved NYC landmark in the East Village and I’ve been buying books there most of my life. We're all about big moments in intimate settings, or as we like to say 'big events, small spaces'. I was sad about it and asked if she would call the Strand. A little dose of party fun to break up those boring work emails. My publisher told me that only “big names” get to do a NYC stop, because people don’t show up in Manhattan otherwise. They would only book my New York stop on Long Island. Still, I will look back at this moment as one of my finest.īack when I was planning my book tour, Barnes and Noble wouldn’t book me in NYC. My book did fine, but was not a life changing success story by any means. This was my view from the podium at the Strand the first stop on my book tour in 2016. ![]() ![]() ![]() This can also be done in C1 but with more effort and C1 is my preferred raw developer. ![]() The only benefit I see is DXO's alternate way of modifying mid-levels and their SmartLight. Even using their sample images, I was able to clean up the residual noise in the DXO-processed files. ![]() The noise reduction in DXO is not as flexible as Topaz's AI Clear or DenoiseAI. I even bought DXO PhotoLab4 for $119 (50% off on their Black Friday special) after demo'ing for 3 days. They also offer a 30 day free trial as well. Check out their website for the details and samples. I just popped for the DXO, Nik and Viewpoint programs to correct perspective. Their price deals right now are really something. The NIK plugin's offer a plethora of powerful capabilities such as sharpening, color ranges, black and white conversions and more. It's rather well known and accepted the NR of the DXO Prime is legions beyond anything out there. Which I must say as a package (DXO 4 and NIK plugins) spanks anything Topaz. I have used the Topaz line and abandoned it for the new DXO 4. And since Topaz is not restricted to use with only Canon cameras, the question is better asked on the Post-processing & Printing Forum, even if you only use Canon gear.Īnd here you are asking for quickie FM reviews when you say But reading online reviews has me conflicted Well if you learn how to use the Search feature on this site, you will find it has been discussed many times. What Topaz product(s) do you use, and how do you like it?. I use AI Clear most of the time, and AI DeNoise some of the time. ![]() They also offer a 30 day free trial as well.Īlso AI clear is fast to use from lightroom. Haven't used any of the others.The work together with lightroom. In De noise is AI clear which is great for most photos and sharpens a little also.AI clear works well in auto mode and the sharpening is subtle and not overdone. I use topaz AI De noise and topaz AI sharpen. What Topaz product(s) do you use, and how do you like it? I plan on downloading the trials to a couple of these products, but with work and other obligations I won't be able to try everything out before the sale deadline hence, this question: I am wondering if the 'pros' using the software are just pushing it because they get a slice of the pie whenever someone purchases software using a promo code linked to their name. While some swear by Topaz, others remark that only gullible newbies get suckered into buying the software. I have been waiting for this sale for months, as I hear quite a bit about Sharpen AI and DeNoise AI, and a bit here and there on the other products.īut reading online reviews has me conflicted. Right now the company is offering a huge bundle (7 products) for $199 ($170 after promo code.about 25 bucks per plug-in). Okay, dumb question, as I am sure enough of you have and perhaps still do use Topaz Labs. ![]() ![]() JSON Type Definition only supports tagged unions, so unions in JTD are fully supported for JTDSchemaType and JTDDataType. This example uses the same data and schema types as above: # Type-safe unions With typescript, your compiled parsers and serializers can be type-safe, either taking their type from schema type or from type parameter passed to compilation functions. With both JSON Schema and JSON Type Definition, the validation error type is an open union, but it can be cast to tagged unions (using validation keyword as tag) for easier error handling.Ĭontinuing the example above: # Type-safe parsers and serializers This is accurate, but often requires extra validation on the part of the user to confirm they're getting the appropriate data type. Note that it's currently not possible for JTDDataType to know whether the compiler is inferring timestamps as strings or Dates, and so it conservatively types any timestamp as string | Date. You can use JTD schema to construct the type of data using utility type JTDDataType See this test (opens new window) for an advanced example. when utility type is used, compiled JTD serializers only accept data of correct type (as they do not validate that the data is valid) and compiled parsers return correct data type.įor the same example as in Getting started:.validation errors for JSON Schema are defined as tagged unions, for type-safe error handling. ![]() compiled validation functions are type guards that narrow the type after successful validation.utility type JTDDataType to convert JSON Type Definition schema into the type of data that it defines.utility types JSONSchemaType and JTDSchemaType to convert data type into the schema type to simplify writing schemas, both for JSON Schema (but without union support) and for JSON Type Definition (with tagged unions support).Ajv takes advantage of TypeScript type system to provide additional functionality that is not possible in JavaScript: ![]() ![]() ![]() According to Polly she stole her video idea, format and intro. Both have things the other wants: Marriage on Brooke's part, and youth, beauty and success on Polly's.Ĭlarissa is a rival TeleYouber to Polly. However, Brooke disagrees with how Polly views marriage, and in response to her saying that she wants to be a "cute little wifey," Brooke sighs and mutters, "To be so naive and idealistic again." In return, Polly wonders how Brooke is able to be so far away from her husband. Though at first she seems put off by Zoey's behavior, Zoey asks Polly about her troubled past and offers her compassion, to Polly's gratitude.īrooke and Polly get along well generally. Polly first notices Zoey due to her odd fashion choices, and her attempts at giving Zoey makeup advice only lead to awkwardness. The two have been mutual fans for some time and get along great. Polly comes to Inna de Poona to do a photo shoot with Ashley. Once she does, though, she remarks that Polly has changed a lot since the last time they saw each other, though Candace's terrible memory wouldn't make this very surprising. She recognizes her right away, though Candace herself has a bit of a hard time remembering who Polly is. Polly used to babysit Candace when she was younger. However, Polly still holds some level or resentment or at least emotional pain over the intense bullying she suffered when she was younger. Polly is not mentioned to, nor mentions herself, having any parents or siblings and the nature of any of her familial relationships is highly speculative. Given Candace's mental faculties even as an adult, however, it can also be assumed she needed care even at an age when most children can be by themselves. Polly also babysat Candace when she was younger, and from their conversations they had a very good relationship and still think well of each other, though this leads to some potential loopholes in the story because Polly is only about seven years older than Candace, which means that Polly may have been either quite a young teenager or preteen when she began babysitting Candace. as outdated), potentially her being transgender, or both. ![]() Polly was frequently bullied growing up for what remains ambiguous reasons, though it can speculated it was for being a very flamboyant and "strange" person (seeing her life goals, fashion sense, etc. Zoey mentions that Polly has something of a troubled past, and clues of Polly's sad childhood can be gleamed in her conversations with Candace, who she used to babysit. Polly's unique gift type are Antiques and her shoe type are Pumps. Polly will drain 5% of the total Affection Goal whenever her date receives a date gift, unless she's upset or exhausted.Ĭannot use any cosmetic or jewelry gifts unless Polly is upset or exhausted.Īll of the 12 dateable girls have two sets of four unique gifts each, in charge of increasing the player's Passion and Style LVL. If Polly becomes upset, she'll drain 4 sentiment from her and her date. Despite her few negative attributes, including her intense jealousy of other women when too much attention is put on them and not her, Polly is a very sweet, good-humored lady with a load of both sass and class.īaggage is a feature exclusive to HuniePop 2 that equips all 12 dateable girls with a set of three unique traits that are unlocked during conversation and pose additional challenge during dates. She also runs a highly successful beauty channel online, and can be highly catty and competitive with her contemporaries. Polly is overtly a 'girly-girl', enjoying stereotypically feminine activities involving makeup, fashion and gossip. Her idyllic future is to become a 1950s-style traditional housewife and make her husband (if the Player is male) or wife (if the Player is female) happy. Polly is a classy girl who absolutely believes she was born in the wrong decade. You're gonna need it!" - Kyu via Hunie Direct - Aug 2018 video. Also, she has a dick! So you better pack some extra lube in that carry-on. ![]() She even has her own online beauty channel with like tons of viewers. ![]() She's kinda obsessed with stereotypically girly things like fashion and makeup. She's a very classy and traditional lady that dreams of someday being the perfect housewife. "Polly is by far the most requested type of character we've ever had. Along with the headband she wears chunky, light pink, triangle shaped, dangle earrings, a turquoise leotard with a red and yellow shooting star design, a pink, off-the-shoulder, three quarter length, crop top with a yellow and silver 80's textile design, black arm warmers, and thin, black tights. Polly's default outfit is modeled after 80's clothing. Her makeup consists of pink eyeshadow, blue eyeliner, strawberry colored lipstick, and baby pink nail polish. Polly has a thin, toned body, with moderately sized breasts. She has light skin with a beauty mark on her right cheekbone, turquoise eyes and permed, dyed, blonde hair swept up in a pink headband. ![]() ![]() ![]() You can use mvn dependency:tree to visualize the dependency tree. Please refer to this post for how Maven chooses the closest dependency: I ended up having to add C 2.0 to my pom.xml so that it can be chosen over C 1.0. ![]() C 2.0 is indeed installed and your IDE knows about it, but it's just ignored when running the application. Now the weird thing is: if you navigate the code of B in your IDE and try to jump to the class that only exists in C 2.0, it works correctly. As a result, when B tries to use the class that only exists in C 2.0, a ClassNotFoundException is thrown.However, A is "closer" in the dependency tree, so Maven uses C 1.0 for both A and B and doesn't even warn you about this (it's quite astounding to me).B makes use of a class that only exists in C 2.0.Let's say A depends on C 1.0 and B depends on C 2.0 The pages below will provide additional details about these errors. ![]() These errors can be a helpful debugging aid, but the reported problem isn't always immediately clear. ![]()
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